文献类型：期刊文章

作　　者：孙璐[1,2] 刘瑜洁[1,2] 仰浈臻[1,2] 齐宪荣[1,2,3]

机构地区：[1]北京大学医学部分子药剂学与新释药系统北京市重点实验室,北京100191 [2]北京大学医学部药学院药剂学系,北京100191 [3]北京大学医学部天然药物及仿生药物国家重点实验室,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》

基　　金：National Natural Science Foundation of China(Grant No.81273454 and 81473156);Beijing Nature Science Fo undation(Grant No.7132113);Doctoral Foundation of the Ministry of Education(Grant No.20130001110055);National Key Ba sic Research Program(Grant No.2013CB932501)

年　　份：2015

卷　　号：24

期　　号：11

起止页码：712-720

语　　种：中文

收录情况：CAB、CAS、CSCD、CSCD2015_2016、DOAJ、EMBASE、IC、JST、SCOPUS、ZGKJHX、普通刊

摘　　要：The epidemiological statistics reveals the striking patterns of cancer in women and highlights the need for novel therapeutic strategies. In this work, mesoporous silica nanoparticles（MSNs） as representative of inorganic nanoparticles were prepared for loading si RNA that plays a role of gene silencing to treat breast carcinoma（MCF-7） cells. The critical processes of synthesis were optimized for the nanoparticles with desired quality attributes that have the enlarged pores for elevated loading capacity. After si RNA loading into mesoporous, crosslinked-polyethylenimine was employed as the cap to coat the enlarged MSN pores and protect the cargo from leakage. The elevated quantity of si RNA（35 μg si RNA/mg MSNs） were loaded in the MSNs. The as-synthesized MSNs were further evaluated on MCF-7 cells in vitro and shown negligible cytotoxicity. As expected, the si RNA loaded in the as-synthesized MSNs was readily internalized into MCF-7 cells and displayed 420 times higher intake than that of naked si RNA. The MSNs may be exploited to become an effective si RNA cell delivery strategy and further studied for the anti-tumor efficacy.

关 键 词：Mesoporous silica nanoparticle  PREPARATION POLYETHYLENIMINE MCF-7 cells  

分 类 号：R318[生物医学工程类] O613.72[基础医学类]