文献类型：期刊文章

作　　者：李晓红[1] 李俊杰[2] 张海伟[3] 孙鹏[1] 张艳玲[1] 蔡绍晖[1] 任先达[1]

机构地区：[1]暨南大学药学院药代动力学教研室 [2]附属华侨医院临床药理研究室 [3]医学院病理教研室

出　　处：《Acta Pharmacologica Sinica》

基　　金：Project supported by the Overseas Chinese Affairs Office of the State Council Foundation, № 39770300.

年　　份：2003

卷　　号：24

期　　号：10

起止页码：1045-1050

语　　种：中文

收录情况：AJ、BDHX、BDHX2000、BIOSISPREVIEWS、CAB、CAS、CSA、CSA-PROQEUST、CSCD、CSCD2011_2012、EBSCO、EMBASE、FSTA、IC、JST、PROQUEST、PUBMED、SCI-EXPANDED(收录号：WOS:000185749400016)、SCIE、SCOPUS、WOS、ZGKJHX、核心刊

摘　　要：AIM: To investigate whether nimesulide could suppress tumor growth and induce apoptosis in implanted hepatoma mice and to explore the molecular mechanisms. METHODS: Male mice received nimesulide 10mg/kg, 20mg/kg, and 40mg/kg ig daily for 21d. Electron microscopy(EM), flow cytometry(FCM), DNA ladder, radioimmunoassay(RIA), and Western blot analysis were employed to investigate effect of nimesulide on mice hepatoma and the related molecular mechanisms. RESULTS: Nimesulide inhibited the growth of hepatoma(from 14% to 62%) and elicited typical apoptotic morphologic changes. The DNA ladder of high dose nimesulide was more clearly observed and apoptotic rate was 51.3%±1.5%. Nimesulide also decreased cyclooxygenase-2(COX-2), prostaglandin E_2(PGE_2) and Bcl-2 expression, while increased the level of Bax protein. CONCLUSION: Nimesulide supresses tumor growth and induces apoptosis by inhibiting COX-2 and PGE_2 expression, which may be related to overexpression of Bax over Bcl-2.

关 键 词：NIMESULIDE cyclooxygenase inhibitors  PROSTAGLANDINS apoptosis  liver neoplasms  

分 类 号：R735.7] R971.1[临床医学类]